Excited to see that our customer, EPFL, has developed a new pipeline named parSEQ. This pipeline, designed with the help of CYTENA's B.SIGHT, aims to optimize the capture of sequence-function datasets from screened protein variant pools. With parSEQ, the functional data of each screened variant is effectively paired with its corresponding sequence data.
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Our lab uses the B.SIGHT to sort microbial cells from environmental samples. It has a very intuitive user interface and the product managers at CYTENA have been very helpful in optimizing the parameters to suit our needs. It dispenses single cells gently with precision and can fill a 384-well plate in under an hour. The sorting speed and accuracy has greatly maximized our capacity to create large libraries of pure cultures.

Single cell efficacy: 70–93%; Colony efficacy: 58–91% (after optimization) [...] The plating effort (to get single cells) can be lowered compared to limited dilution and FACS single cell deposition by the factor 4-5 [...]

When using the single-cell printer, the number of monoclonal cell lines increased 6-fold compared to the limiting dilution process, therefore increasing the number of pools that could be cloned… By implementing both of these technologies, the cell line development process can be decreased from 9 months to 6 months.

[CYTENA’s] cloning platform is suitable for generating clonal cell lines with a probability of ≥99.9%... Overall, the implementation of this novel technology provides significant advantages over traditional methods with respect to cost, simplicity, timing and flexibility.